Interspecific fusion of bovine and other cells with parainfluenza viruses (Sendai and Pi-3).
نویسندگان
چکیده
The artificial fusion of cells of different type and species, for the formation of heterokaryons and hybrid cells, is now widely used, since Harris and Watkins (1965) demonstrated the practicality of this technique, with Sendai virus as a fusing agent. A variety of viruses show fusing properties (Poste, 1972)) but in most artificial systems Sendai virus (parainfluenza virus type 1) has been used, because of the wide range of cells which contain surface receptor sites for it (Harris, 1970). However, cells do vary in their fusing response to Sendai virus (Okada and Tadokoro, 1963), and Irvin, Brown. Stagg, Kanhai and Rowe (1975a) and Irvin, Stagg and Kanhai (1976) obtained disappointing results with Sendai virus in bovine/hamster and bovine/mouse fusion systems in that fusion percentage was low and no hybrid lines were developed. Consequently it was decided to investigate the use of a virus with more specific affinity for bovine cells as an alternative fusing agent. Omar (1965) showed that bovine parainfluenza virus type 3 (Pi-3) caused extensive intraspecific fusion of bovine and other cells in vitro and Kita and Gillespie ( 1968) examined the susceptibility of various cell types to bovine Pi-3 virus, noting fusion in a number of cases. The current study compares the fusing properties of Sendai and Pi-3 in a variety of interspecific cell systems with particular emphasis on bovine cells.
منابع مشابه
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ورودعنوان ژورنال:
- Journal of comparative pathology
دوره 87 3 شماره
صفحات -
تاریخ انتشار 1977